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In practice, this technique generates a mixed population of 3D and monolayer cells and is not ideal for redifferentiation applications The aggregate model involves culturing chondrocytes in nonadherent wells or dishes These can be purchased (Corning-Costar ultralow-attachment plates) or made by layering the bottom of regular dishes with methacrylate [Reginato et al, 1994; Stokes et al, 2002] or agarose [Archer et al, 1990; Kolettas et al, 1995] The aggregate model is ideal for maintaining the differentiated phenotype of primary chondrocytes It is less effective for restoring the differentiated phenotype of monolayer-expanded chondrocytes because the characteristic aggregation that occurs with primary cells is attenuated after monolayer expansion For this application, the pellet model (Protocol 44) is more effective Alginate [Bonaventure et al, 1994; Gagne et al, 2000; H uselmann et al, a 1994; Homicz et al, 2003; Mandl et al.

In using such electrical analog models, groundwater ow was quanti ed by means of a direct comparison between Darcy s Law and Ohm s Law (which equates electrical current to the ratio of voltage to resistance) Although such electrical analog models have been almost wholly superseded by digital models now, it is important not to lose sight of the key fact that groundwater ows in a manner entirely analogous to heat transfer and electromagnetic conduction At root, the reason why groundwater movement occurs in ow elds comes back to the laminar nature of most subsurface ow With no turbulent eddy currents and negligible momentum, groundwater tends to move in a very smooth manner, gently maintaining continuity between the recharge area in which it rst seeped below the water table and the discharge zone, through which it will eventually leave the aquifer.

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Oracle Lock Escalation Oracle automatically converts a table lock of lower restrictiveness to one of higher restrictiveness as appropriate. As an example, assume a transaction issues a SELECT . . . FOR UPDATE statement. In this case, the transaction acquires an exclusive row lock and a row share table lock on the table. If this transaction later updates one or more of the locked rows, the row share table lock is automatically converted to a row exclusive table lock by Oracle. As another example, if a transaction holds many row locks on a table, the database will automatically escalate the row locks to a single table lock for the transaction. Oracle Lock Compatibility Matrix Figure 5.21 summarizes the lock types and compatibilities that are supported by Oracle 10 g.

, 2002; Masuda et al, 2003; Murphy and Sambanis, 2001], agarose [Benya and Shaffer, 1982; Lee et al, 1998; Weisser et al, 2001], brin [Fortier et al, 2002; Hendrickson et al, 1994], and collagen [Kuriwaka et al, 2003; Lee et al, 2003] models involve suspension of the chondrocytes within a 3D substrate or gel The substrate-based models are particularly suitable for tissue engineering applications because phenotypic redifferentiation and generation of an engineered construct for implantation can occur simultaneously [Chang et al, 2001; Marijnissen et al, 2002; Masuda et al, 2003; Miralles et al, 2001; Weisser et al, 2001] The alginate model is particularly versatile in that the chondrocytes can be reisolated from the construct by calcium chelation [Masuda et al, 2003] (Protocol 45)..

Contours of groundwater head, ow lines, ow nets, and ow tubes We have already seen how groundwater head is measured in practice (Box 31) When we have collected a large number of simultaneousviii head measurements from different points in a given groundwater ow system, we are in a position to begin to delineate groundwater ow patterns The rst step is to contour the head values..

advances the Debugger out of a function. This button works only if you are currently stopped in a user-defined function; it moves the yellow arrow to the line after the one where that function was called. In the example above, if you place a breakpoint at line 3 and click Step Out, the Debugger moves to line 8. Clicking Step Out at a line that is not within a user-defined function is the same as clicking Continue. For example, if you stop at line 6 and click Step Out, the player continues executing the script until it encounters a breakpoint.

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